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Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species

Year: 2021
Type: article
Abstract: We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.
DOI: 10.1093/infdis/jiab431
Authors: Camilla Luiza-Batista, Flore Nardella, Sabine Thiberge, Malika Serra-Hassoun, Marcelo U Ferreira, Artur Scherf, Sylvie Garcia
Institutions: Centre National de la Recherche Scientifique, Inserm, Institut Pasteur, Sorbonne Université, Biologie des interactions hôte-parasite, Universidade de São Paulo
Language: English
Field: Medicine
Open Access status: hybrid
Estimated APC paid: $3,550
OpenAlex ID: W3193344099
FWCI: 0.65
Cites: 14
Cited by: 5
Related to: 10