Cellular origins and lineage relationships of the intestinal epithelium
Year: 2021
Type: review
Abstract: Knowledge of the development and hierarchical organization of tissues is key to understanding how they are perturbed in injury and disease, as well as how they may be therapeutically manipulated to restore homeostasis. The rapidly regenerating intestinal epithelium harbors diverse cell types and their lineage relationships have been studied using numerous approaches, from classical label-retaining and genetic lineage tracing methods to novel transcriptome-based annotations. Here, we describe the developmental trajectories that dictate differentiation and lineage specification in the intestinal epithelium. We focus on the most recent single-cell RNA-sequencing (scRNA-seq)-based strategies for understanding intestinal epithelial cell lineage relationships, underscoring how they have refined our view of the development of this tissue and highlighting their advantages and limitations. We emphasize how these technologies have been applied to understand the dynamics of intestinal epithelial cells in homeostatic and injury-induced regeneration models.
Institution: Columbia University Irving Medical Center
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Funders: Burroughs Wellcome Fund, Irma T. Hirschl Trust, Gerstner Family Foundation, New York State Stem Cell Science, National Institute on Aging, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke
Awards: Limited Competition for the Continuation of the Intestinal Stem Cell Consortium Coordinating Center, Directing Cell Fate Along the Intestinal Enteroendocrine Lineage, Predoctoral Training Grant in Genetics and Development, Intestinal Stem Cell Function During Aging and Tumor Initiation, Single Cell Analysis of the Infiltrative Margins of Glioblastoma and Post Treatment Recurrence, Multidisciplinary Training in Translational Gastrointestinal and Liver Research
OpenAlex ID: W3193376981