Year: 2021
Type: article
Abstract: growth conditions to cell media used for toxicity studies induced distinct mesoscopic precipitates, two of which resemble either diffuse or neuritic plaques seen in Alzheimer's histology. Our biophysical characterization indicates that both oligomer species share morphological and tinctorial features considered characteristic for amyloid oligomers. At the same time, their sizes, morphologies, their immunostaining, detailed tinctorial profiles and, most prominently, their biological activity are clearly distinct from each other. Probing the conditions promoting the formation of these two distinct oligomer species suggests distinct roles of charge interactions, hydrophobicity and monomer flexibility in directing oligomer assembly.
Source: RSC Chemical Biology
DOI: 10.1039/d1cb00081k
Authors: Chamani Niyangoda, Jeremy Barton, Nabila Bushra, Kanchana Karunarathne, Graham Strauss, Fadia Fakhre, Piyush Koria, Martin Muschol
Institution: University of South Florida
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,962
OpenAlex ID: W3193570693