Work

A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves

Year: 2021

Type: article

Abstract: A cornea is innervated by sensory nerves, which branch into thick trunks, subbasal plexuses, and sensory endings. Appropriate assessment of nerve structure in a tissue provides a more complete understanding of the role of nerves in health and disease. Here, we present a whole-mount immunohistochemistry protocol that facilitates evaluation of nerve architecture throughout the mouse cornea. The fixation step in this protocol allows for reliable detection of nerve structures within the cornea and likely other tissues. For complete details on the use and execution of this protocol, please refer to Yun et al, (2020).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2021.100734

Authors: Hongmin Yun, Kira L. Lathrop, Anthony J. St. Leger

Institution: University of Pittsburgh

Language: English

Topic: Ocular Surface and Contact Lens

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

Open Access status: gold

Estimated APC paid: $1,500

Funders: Eye and Ear Foundation of Pittsburgh, Research to Prevent Blindness, National Institutes of Health, National Eye Institute