Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence
Year: 2021
Type: article
Abstract: Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ system. Through careful optimization, we ensure high-quality and reproducible datasets suitable for cross-patient comparisons that are essential to understanding human health and disease. Moreover, each of these steps can be adapted to other organ systems or diseases, enabling additional atlas efforts.
Source: STAR Protocols
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Funders: National Science Foundation, Vanderbilt University, National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Environmental Health Sciences, Division of Chemical, Bioengineering, Environmental, and Transport Systems, Common Fund
Awards: G1809294206, Vanderbilt University Biomolecular Multimodal Imaging Center for 3-Dimensional Tissue Mapping, Defining the impact of host factors on the molecular architecture and bacterial physiology of Staphylococcus aureus abscesses, Tennessee Valley Cooperative Human Tissue Network, Molecular mapping of microbial communities at the host-pathogen interface by multi-modal 3-dimensional imaging mass spectrometry, Training Program in Environmental Toxicology, Imaging Mass Spectrometry Research Resource at Vanderbilt University
OpenAlex ID: W3194849030