Work

Application of Relaxation Dispersion of Hyperpolarized 13C Spins to Protein–Ligand Binding

Year: 2021

Type: article

Abstract: Nuclear spin relaxation dispersion parameters are proposed as indicators of the binding mode of a ligand to a protein. Hyperpolarization by dissolution dynamic nuclear polarization (D‐DNP) provided a 13C signal enhancement between 3000–6000 for the ligand 4‐(trifluoromethyl) benzene‐1‐carboximidamide binding to trypsin. The measurement of 13C R2 relaxation dispersion was enabled without isotope enrichment, using a series of single‐scan Carr–Purcell–Meiboom–Gill experiments with variable refocusing delays. The magnitude in dispersion for the spins of the ligand is correlated to the position with respect to the salt bridge between protein and the amidine group of the ligand, indicating the ligand binding orientation. Hyperpolarized relaxation dispersion is an alternative to chemical shift or NOE measurements for determining ligand binding modes.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.202109430

Authors: Chang Qi, Yunyi Wang, Christian Hilty

Institution: Texas A&M University

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: Hyperpolarization Assisted and Structure Based Screening of Protein-Ligand Interactions in Live Cells