Work

Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling

Year: 2021

Type: article

Abstract: A key question is whether PARP1 has a role(s) in ALC1-dependent nucleosome remodeling beyond simply synthesizing the PAR chains needed to activate the ALC1 ATPase. Here, we identify PARP1 separation-of-function mutants that activate ALC1 ATPase but do not support nucleosome remodeling by ALC1. Investigation of these mutants has revealed multiple functions for PARP1 in ALC1-dependent nucleosome remodeling and provides insights into its multifaceted role in chromatin remodeling.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.2107277118

Authors: Soon-Keat Ooi, Shigeo Sato, Chieri Tomomori-Sato, Ying Zhang, Zhihui Wen, Charles A. S. Banks, Michael P. Washburn, Jay R. Unruh, Laurence Florens, Ronald C. Conaway, Joan W. Conaway

Institutions: Stowers Institute for Medical Research, University of Kansas Medical Center

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Stowers Institute for Medical Research

OpenAlex ID: W3197229060

FWCI: 0.549

Cites: 42

Cited by: 19

Related to: 10