Work

Extension of hydrodynamic chromatography to DNA fragment sizing and quantitation

Year: 2021

Type: review

Abstract: Hydrodynamic chromatography (HDC) is a technique originally developed for separating particles. We have recently extended it to DNA fragment sizing and quantitation. In this review, we focus on this extension. After we briefly introduce the history of HDC, we present the evolution of open tubular HDC for DNA fragment sizing. We cover both the theoretical aspect and the experimental implementation of this technique. We describe various approaches to execute the separation, discuss its representative applications and provide a future perspective of this technique in the conclusion section of this review.

Source: Heliyon

DOI: 10.1016/j.heliyon.2021.e07904

Authors: Yanan Wang, Yingyan Zhou, Dongtang Zhang, Xiayan Wang, Shaorong Liu

Institutions: Beijing University of Technology, University of Oklahoma

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $1,750

Funder: National Natural Science Foundation of China