An efficient, short and cost-effective regeneration system for transformation studies of sugarcane (Saccharum officinarum L.).
Year: 2009
Type: article
Abstract: Sugarcane genetic transformation efforts are seriously hampered by the lack of an efficient and reproducible regeneration system. An efficient, short and cost-effective regeneration system, through direct embryogenesis, was developed for local cultivars and elite lines of sugarcane. Using 1-2 mm thick meristematic young leaf whirls, direct embryogenesis was achieved in Murashige & Skoog (MS) medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) under cool white fluorescent light for 16 hour/day at 25 ± 2 °C within three weeks. Of the various concentrations of 2, 4-D tested, 3 mg/L induced the highest frequency of embryogenic callus (60 %). The embryos germinated in the fourth week on the same medium. Shoot proliferation and multiplication was carried out in liquid MS medium containing benzyl aminopurine (BAP) at a concentration of 1 mg/L. The improved regeneration system will particularly be useful in our ongoing genetic transformation studies.
Authors: A. H. Shah, Naeem Rashid, Muhammad Salman Haider, Faiza Saleem, Maimun Binti Tahir, Javed Iqbal
Institution: University of the Punjab
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W319982301