Work

Identification of resistance gene against phytophthora root rot in new soybean lines breeded in Henan province

Year: 2020

Type: article

Abstract: Phytophthora root rot is one of the destructive diseases affecting soybean production, which is a great threat to soybean production. Planting resistant soybean cultivars is the most effective way to control this disease. Henan province was located in the hinterland area of the summer-sowing soybean production region of Huang-Huai in China, which had the potential threat region of phytophthora root rot. The objective of this study was to screen effective resistance cultivars for disease control and resistance breeding by phenotypic identification and molecular detection of resistance gene. Sixty-four new soybean lines bred in Henan were evaluated for their resistance responses to two Phytophthora sojae isolates PsJS2 and Ps41-1 using the hypocotyls inoculation technique. The result showed that 35 lines and 16 lines were resistance to Ps41-1 and PsJS2, respectively. Sixteen lines and 10 lines were intermediate to Ps41-1 and PsJS2, respectively. And there were 16 lines resistance to both Ps41-1and PsJS2, accounting for 25% of tested lines. Sixty-four lines was detected for Phytophthora resistance gene by using molecular marker WZInDel11 co-segregating with a resistance gene RpsZheng. The results showed that, 13 of 16 lines resistant to both PsJS2 and Ps41-1 contain target band of WZInDel11, while 5 lines resistant to one of two P. sojae isolates show segregating to P. sojae produced heterozygous bands. The homozygous resistant plants of these lines segregating for resistance could be accurately detected by marker WZInDel11, and further were directly developed into homozygous resistant lines. Combining the results of pedigree analysis, it was speculated that two lines might contain the resistance gene RpsZheng, two lines might contain RpsYD29, and 14 lines might contain RpsZheng or its allele. In conclusion, the results indicated that the new soybean lines cultivated in Henan Province had excellent resistance sources to P. sojae. This study provides important information for disease control and resistance breeding.

Source: ACTA AGRONOMICA SINICA

DOI: 10.3724/sp.j.1006.2021.04105

Authors: Xue-Cui ZHANG, Su-Li SUN, Wei-Guo LU, Hai-Chao LI, Yan-Yan JIA, Can-Xing DUAN, Zhen-Dong ZHU

Institutions: Chinese Academy of Agricultural Sciences, Institute of Crop Sciences, Henan Academy of Agricultural Sciences, Harbin Institute of Technology

Language: English

Topic: Plant Pathogens and Resistance

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: bronze

Funders: National Natural Science Foundation of China, Shandong Academy of Agricultural Sciences