Effects on C-phycocyanin content of Arthrospira (Spirulina) platensis of culture medium containing geothermal water.
Year: 2021
Type: article
Abstract: This research aimed to compare effects on product quality and algal growth of inoculation ratio of Arthrospira platensis in the culture medium containing 20% geothermal water. A. platensis was inoculated at the ratio of 1/6, 1/10, and 1/20 of nutrient medium volume (2500 mL). The experiment medium was prepared with 20% geothermal water and 80% distilled water. Schlösser medium, 100% geothermal water medium, and 100% distilled water were used as the control group. At the end of the experiment, Spirulina biomass was obtained by filtration through 80-micron plankton cloth and freeze-dried at -60 °C. Increasing inoculation density shortened the culture time and increased the growth rate compared to the other groups. The best growth among the experimental groups was obtained in a 1/6 ratio inoculated Spirulina group in a 20% geothermal water medium. Among the experimental groups, dry biomass was obtained in the Spirulina group inoculated at 1/6 in only 20% geothermal water medium. The optical density value was 0.989 (A750), and the biomass yield was 0.476 g/L in the experimental group, the highest among the Schlösser groups was 1.259 (A750), and the biomass yield was 0.928 g/L. The most efficient growth and phycocyanin content was determined in the 1/6 inoculated groups. The phycocyanin content in the experimental group was found in 22.49%, and the purity rate was 2.24. In control groups, 3.73 purity and 28.62% phycocyanin were determined in the Schlösser medium. While 48.42% protein was detected in the geothermal water group, 61.64% was obtained with the Schlösser medium.
Source: Natural Science and Discovery
DOI: 10.20863/nsd.v4i3.61
Authors: Sibel Bayıl Oğuzkan, Betül Kut Güroy
Institutions: Gaziantep University, Yalova University
Language: English
Field: Energy
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W3202388186