Purification and cryo-EM structure determination of Arabidopsis thaliana GLR3.4
Year: 2021
Type: article
Abstract: Ionotropic glutamate receptors (iGluRs) are ligand-gated ion channels that play crucial roles in the central nervous system. iGluR homologs, termed glutamate receptor-like channels (GLRs), have been found in plants. Investigating the structural and functional relationship between iGluRs and GLRs was limited by GLR protein expression, purification, and structural characterization. Here, we provide a detailed protocol for Arabidopsis thaliana GLR3.4 ( At GLR3.4) expression in a mammalian cell line and purification for structure determination by cryogenic electron microscopy (cryo-EM). For the complete details on the use and execution of this protocol, please refer to Green et al. (2021).
Source: STAR Protocols
Institution: Columbia University
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,500
Awards: NSF/MCB-BSF: Collaborative Research: Towards development of the structural determinants of the Glutamate receptor gating regulation by auxiliary membrane anchored proteins, G2390790659, G6099013130, G6372839752, G686058816, G7986127690, G830600299, Structure and function of Transient Receptor Potential Channels, Structural and functional principles of activation and regulation of the transient receptor potential channel TRPV3.
OpenAlex ID: W3202644302