Work

Modulation of Transaminase Activity by Encapsulation in Temperature‐Sensitive Poly(N‐acryloyl glycinamide) Hydrogels

Year: 2021

Type: article

Abstract: Smart hydrogels hold much potential for biocatalysis, not only for the immobilization of enzymes, but also for the control of enzyme activity. We investigated upper critical solution temperature-type poly N-acryloyl glycinamide (pNAGA) hydrogels as a smart matrix for the amine transaminase from Bacillus megaterium (BmTA). Physical entrapment of BmTA in pNAGA hydrogels results in high immobilization efficiency (>89 %) and high activity (97 %). The temperature-sensitiveness of pNAGA is preserved upon immobilization of BmTA and shows a gradual deswelling upon temperature reduction. While enzyme activity is mainly controlled by temperature, deactivation tended to be higher for immobilized BmTA (≈62-68 %) than for free BmTA (≈44 %), suggesting a deactivating effect due to deswelling of the pNAGA gel. Although the deactivation in response to hydrogel deswelling is not yet suitable for controlling enzyme activity sufficiently, it is nevertheless a good starting point for further optimization.

Source: ChemBioChem

DOI: 10.1002/cbic.202100427

Authors: Katrin Kappauf, Nikola Majstorovic, Seema Agarwal, Dörte Rother, Christiane Claaßen

Institutions: Forschungszentrum Jülich, RWTH Aachen University, Bavarian Polymer Institute, University of Bayreuth

Language: English

Topic: Hydrogels: synthesis, properties, applications

Subfield: Molecular Medicine

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,800

Funders: TU Graz, Internationale Beziehungen und Mobilitätsprogramme, European Commission, H2020 European Research Council