High resolution estimates of relative gene abundance with quantitative ratiometric regression PCR (qRR-PCR)
Year: 2021
Type: article
Abstract: method, our qRR-PCR algorithm leverages the innate relationship of co-amplified PCR targets to measure their relative quantities using characteristic curves derived from the normalized ratios of qPCR fluorescence curves. We demonstrate the utility of this technique for discriminating the fractional abundance of mixed alleles with resolution below 5%.
Source: The Analyst
DOI: 10.1039/d1an01397a
Institutions: Johns Hopkins University, Corteva (United States)
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3203453680