Development of a Multimodal Imaging Probe for Lymph Node Targeting Using Specific Amphiphile Encapsulation Technology on Iron Oxide Nanoparticles
Year: 2014
Type: dissertation
Abstract: Introduction: Multimodal imaging probes could provide synergistic effect through combining the benefits and supplementing the weaknesses from each imaging system. Nanoparticle is a good carrier and imaging probe itself and surface modification using huge surface area make it possible for multifunctional application. The aim of this study is to develop a multimodal iron oxide nanoparticle using an encapsulation method with specific amphiphiles (1).\nMethods: Specific amphiphiles were synthesized by conjugating stearylamine with SCN-Bn-NOTA and SCN-Bn-Mannose. Mannose-containing nanoparticle was designed for imaging lymph nodes (2). NOTA is an excellent chelating agent for 68Ga labeling (3). Iron oxide nanoparticles were encapsulated with prepared solution containing polysorbate 60 and functionalized amphiphiles by vigorous mixing and sonication. Reaction mixture was purified using size exclusion column and concentrated with centrifugal filter. Concentration of the final material was measured by colorimetric analysis at 457 nm. Size distribution was analysed using a DLS instrument. Stability was evaluated under various conditions (0.9, 1.8, 3.6% NaCl solution, rt, 1, 3, 24 h
Author: 양보연
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W3203664848