Association Studies of Different Genetic Variants of αS2-casein Gene (CSN1S2) With Milk Production Traits (Lactose, SNF&Density) in Malvi, Nimari, Sahiwal & HF Crossbred Cattle
Year: 2021
Type: preprint
Abstract: αs2-casein is a primary milk protein. However, there has been little research focused on the effects of αs2-casein variants on milk. The αs2-casein (CSN1S2) play a major protein found in ruminant’s milk, which is encoded by a highly polymorphic CSN1S2 gene present on chromosome 6. Research work was carried out on 50 animals each of Malvi, Nimari, Sahiwal and HF Crossbred cattle at Jabalpur, Madhya Pradesh, India. The αS2 casein gene (1267 bp) was digested by EcoRV restriction enzyme yielding two genotypes viz., AA (1267 bp) and BB (1267/1150/117 bp) in Malvi, Nimari and HF crossbred cattle, while only one genotype (AA) in Sahiwal. The restriction site was absent in Sahiwal resulting in only single compact band of 1267 bp. The tested animals of Sahiwal were found monomorphic at this locus. Association study of different genotypes with milk composition traits revealed that the mean lactose % was significantly higher in Malvi compared to others for AB genotype animals as compared to AA genotype. The mean SNF % between AA and AB was found non-significant in Malvi, Nimari and HF crossbred. The mean milk density (kg/L) was significantly higher for AB genotype than AA genotype of Nimari and HF crossbred. The mean milk density (kg/L) was observed maximum in Nimari for AB genotype and minimum in Malvi and Sahiwal for AA genotype. The genotypic and gene frequencies of αS2-casein gene (CSN1S2)/EcoRV locus pattern showed that the frequencies of AA, AB and BB genotypes were found to be 0.44, 0.56 and 0.00 in Malvi; 0.68, 0.32 and 0.00 in Nimari; 1.00, 0.00, and 0.00 in Sahiwal and 0.58, 0.42 and 0.00 in HF crossbred, respectively. The frequency of A allele was found to be highest as compared to B allele in all the four breeds of cattle under the study.
Source: Research Square
Authors: Akhilesh Pandey, Zeeshan Khan
Language: English
Subfield: Genetics
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W3205255440