Compared to Traditional Extract, Moringa oleifera Leaf Extract Nanoparticles Effectively Boost the Performances and Antioxidant Defense Systems in Cadmium-Stressed Phaseolus vulgaris Plants
Year: 2019
Type: article
Abstract: Phaseolus vulgaris (L.) is cadmium (Cd 2+ )-sensitive crop that needs an exogenous support to grow well under Cd 2+ stress.Therefore, it was grown in the presence of 1 mM Cd 2+ beginning from 15 days after sowing (DAS) and was foliar-sprayed with Moringa oleifera leaf extract nanoparticles (n-MLE) in comparison to traditional extract (t-MLE) at 20 and 30 DAS.Plants were sampled for growth, physio-biochemical, antioxidant analysis, and yield.The optimized levels of 2 and 6% of n-MLE and t-MLE, respectively from the preliminary study were used.Growth and yield attributes, levels of leaf chlorophylls and carotenoids, plant water use efficiency (WUE), photosynthesis efficiency in terms of PI and Fv/Fm, cellular relative water content (RWC) and cellular membrane stability (MSI) were significantly decreased.In contrast, Cd 2+ content in different plant parts, ion leakage (EL), peroxidation of lipids (MDA content), content of hydrogen peroxide (H 2 O 2 ), free proline, soluble sugars, α-tocopherol (αTOC), glutathione (GSH), ascorbic acid (AsA), and activity of glutathione peroxidase (GPX), ascorbate peroxidase (APX), catalase (CAT), and superoxide dismutase (SOD) were markedly elevated with exposing plants to Cd 2+ stress compared with the corresponding control.However, foliar spray with n-MLE or t-MLE considerably detoxified the Cd 2+ stress effects and significantly improved the abovementioned parameters by significantly increasing growth and yield attributes, photosynthetic pigment levels, Fv/Fm, PI, WUE, RWC, and MSI, and further elevating the levels of αTOC, free proline, soluble sugars, AsA, GSH, and the activities of SOD, CAT, APX, GPX.In contrast, Cd 2+ , EL, MDA and H 2 O 2 contents were markedly reduced compared to the corresponding controls.In comparison to t-MLE, n-MLE was more effective treatment that is recommended to use for ameliorating the harmful effects of Cd 2+ stress up to the level of 1 mM.
Author: Saad M. Howladar
Institution: University of Jeddah
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W3205622646