ITIH5 shows tumor suppressive properties in cervical cancer cells grown as multicellular tumor spheroids.
Year: 2021
Type: article
Abstract: tumors more closely. We successfully established CC cell line-derived MCTS using the hanging-drop technique. ITIH5 was ectopically overexpressed in HeLa and SiHa cells and its functional relevance was investigated under three dimensional (3D) culture conditions. We found that ITIH5 re-expression significantly suppressed tumor spheroid growth and spheroid invasiveness of both HeLa and SiHa spheroids. Immunohistochemical (IHC) analyses revealed a significant reduction in Ki-67 cell proliferation index and CAIX-positive areas indicative for hypoxia and acidification. Furthermore, we observed an increase in cPARP-positive cells suggesting a higher rate of apoptosis upon ITIH5 overexpression. An effect of ITIH5 expression on the susceptibility of cervical MCTS towards cytostatic drug treatment was not observed. Collectively, these data uncover pronounced anti-proliferative effects of ITIH5 under 3D cell culture conditions and provide further functional evidence that the downregulation of ITIH5 expression during cervical carcinogenesis could support cancer development.
Source: PubMed
Authors: Ann-Kathrin Daum, Jessica Dittmann, Lars Jansen, Sven Peters, Uta Dahmen, Julia I Heger, Felix Hoppe-Seyler, Alexandra Gille, Joachim H Clement, Ingo B Runnebaum, Matthias Dürst, Claudia Backsch
Language: English
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W3206348507