Biological controls for standardization and interpretation of adaptive immune receptor repertoire profiling
Year: 2021
Type: review
Abstract: Use of adaptive immune receptor repertoire sequencing (AIRR-seq) has become widespread, providing new insights into the immune system with potential broad clinical and diagnostic applications. However, like many high-throughput technologies, it comes with several problems, and the AIRR Community was established to understand and help solve them. We, the AIRR Community's Biological Resources Working Group, have surveyed scientists about the need for standards and controls in generating and annotating AIRR-seq data. Here, we review the current status of AIRR-seq, provide the results of our survey, and based on them, offer recommendations for developing AIRR-seq standards and controls, including future work.
Source: eLife
DOI: 10.7554/elife.66274
Institutions: University of Zurich, University Children's Hospital Zurich, Center for Regenerative Therapies Dresden, Technische Universität Dresden, Sorbonne Université, Assistance Publique – Hôpitaux de Paris, Pitié-Salpêtrière Hospital, Immunologie - Immunopathologie - Immunothérapie, Emory University, University of Pennsylvania, University of Genoa, Vanderbilt University Medical Center, University of Copenhagen, University of Illinois Urbana-Champaign, Carl R. Woese Institute for Genomic Biology, Centre National de la Recherche Scientifique, Université de Montpellier, Institut de Génétique Humaine, Takara (United States)
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
Funders: Massachusetts Department of Fish and Game, Deutsche Forschungsgemeinschaft, Agence Nationale de la Recherche, Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, National Institutes of Health, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, H2020 Fast Track to Innovation, FP7 Ideas: European Research Council
Awards: iMAP, G1752498933, University of Pennsylvania Clinical Autoimmunity Center of Excellence, G2140063125, G2180887151, G2462971813, Functional characterisation of the B cell compartment in children with antibody deficiency and following bone marrow transplantation, G2647246237, Tissue compartmentalization of human lymphocytes, Penn integrated Human Pancreas procurement and Analysis Program, G5271212076, Sorbonne Universités à Paris pour l'Enseignement et la Recherche, Adaptive immune responses in the wounded heart: novel diagnostic and therapeutic opportunities, G6756318152, Characterization of human thymic B cells, G9000026659, Abramson Cancer Center Support Grant
OpenAlex ID: W3165355346