Effective discrimination of gas-phase peptide conformers using TIMS-ECD-ToF MS/MS
Year: 2021
Type: article
Abstract: -isomerizations at X-Pro peptide bond, following the same protonation schemes, in good agreement with previous ion mobility and single point mutation experiments. The comparison between ion mobility selected ECD spectra and traditional FT-ICR ECD MS/MS spectra showed comparable ECD fragmentation efficiencies but differences in the ratio of radical (˙)/prime (') fragment species (H˙ transfer), which were associated with the differences in detection time after the electron capture event. The analysis of model peptides using online TIMS-q-EMSToF MS/MS provided complementary structural information on the intramolecular interactions that stabilize the different gas-phase conformations to those obtained by ion mobility or ECD alone.
Source: Analytical Methods
DOI: 10.1039/d1ay01461g
Authors: K. Jeanne Dit Fouque, M. Wellmann, D. Leyva Bombuse, M. Santos-Fernandez, Y. L. Cintron-Diaz, M. E. Gomez-Hernandez, D. Kaplan, V. G. Voinov, F. Fernandez-Lima
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: Practical Mass Spectrometer Upgrade for Identifying Fragile Protein Modifications by ECD, G1209676841, Development of Multidimensional, Linear and Differential Ion Mobility MS Separations for Middle-Down Proteoforms, Practical Mass Spectrometry Upgrade for Identifying Fragile Protein Modifications by ECD, G6430264209, G8563301941
OpenAlex ID: W3207046339