Year: 2021
Type: article
Abstract: Microcrystal electron diffraction (MicroED) permits structure determination from nano/microcrystals using a cryoelectron microscope, bypassing the need for large crystals that are especially challenging to obtain for many samples. Furthermore, microcrystals improve the efficiency of ligand soaking, allowing for rapid structure determination of multiple drug-protein complexes using the same crystalline sample. As such, MicroED is a valuable technique for drug discovery. Here, we summarize the process of protein-ligand structure determination by MicroED. We also present several structures solved by our lab, which showcase the versatility of MicroED and its potential for structure-based drug design targeting both soluble and membrane proteins.
Authors: Lisa Clark, Larry Andrews, Jean Jakoncic, Robert Sweet, Herbert Bernstein, Dima Kozakov
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W3208395693