Human retinal organoids release extracellular vesicles that regulate gene expression in target human retinal progenitor cells
Year: 2021
Type: article
Abstract: The mechanisms underlying retinal development have not been completely elucidated. Extracellular vesicles (EVs) are novel essential mediators of cell-to-cell communication with emerging roles in developmental processes. Nevertheless, the identification of EVs in human retinal tissue, characterization of their cargo, and analysis of their potential role in retina development has not been accomplished. Three-dimensional retinal tissue derived from human induced pluripotent stem cells (hiPSC) provide an ideal developmental system to achieve this goal. Here we report that hiPSC-derived retinal organoids release exosomes and microvesicles with small noncoding RNA cargo. EV miRNA cargo-predicted targetome correlates with Gene Ontology (GO) pathways involved in mechanisms of retinogenesis relevant to specific developmental stages corresponding to hallmarks of native human retina development. Furthermore, uptake of EVs by human retinal progenitor cells leads to changes in gene expression correlated with EV miRNA cargo predicted gene targets, and mechanisms involved in retinal development, ganglion cell and photoreceptor differentiation and function.
Source: Scientific Reports
Authors: Jing Zhou, Miguel Flores-Bellver, Jianbo Pan, Alberto Benito-Martin, Cui Shi, Onyekwere Onwumere, Jason Mighty, Jiang Qian, Xiufeng Zhong, Tasmim Hogue, Baffour Amponsah-Antwi, Linda Einbond, Rajendra Gharbaran, Hao Wu, Bo-Juen Chen, Zhiliang Zheng, Tatyana Tchaikovskaya, Xusheng Zhang, Hector Peinado, Maria Valeria Canto-Soler, Stephen Redenti
Institutions: The Graduate Center, CUNY, Lehman College, City University of New York, University of Colorado Anschutz, University of Colorado Denver, Johns Hopkins University, Johns Hopkins Medicine, Cornell University, Weill Cornell Medicine, Sun Yat-sen University, State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Bronx Community College, New York Genome Center, Albert Einstein College of Medicine, Spanish National Cancer Research Centre
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,990
OpenAlex ID: W3126631659