S93 Correlation of eotaxin-3 gene expression and other IL-13-induced genes in patients with asthma
Year: 2021
Type: conference-abstract
Abstract: Background Eotaxin-3 is an eosinophilic chemokine. We investigated how its expression in the bronchial epithelium correlates with other IL-13 signature genes in asthma. Methods We performed a post-hoc analysis of data in patients with asthma and healthy controls (PMID#28933920). Asthma inflammatory phenotype was determined by the induced sputum differential: eosinophilic >3%; neutrophilic >61%. Bronchial epithelial gene expression was measured using RNA microarrays on endobronchial brushes. Eotaxin-3 gene expression was compared between phenotypes by one-way ANOVA corrected for 10 comparisons. Pearson correlations (p<0.05 significant) were computed between eotaxin-3 and fourteen other IL-13-induced genes reported in in vitro epithelial cell studies. Results Data from 38 asthmatics and 18 controls were included. Eotaxin-3 gene expression was highest in eosinophilic asthma (figure 1A). The correlation matrix (figure 1B) shows strong associations between eotaxin-3 gene expression and IL-13-induced-genes in patients with asthma. The 3 most correlated genes were Serpin Family B Member 10 (SERPINB10: r=0.85), periostin (POSTN: r=0.79) and nitric oxide synthase 2 (NOS2: r=0.72). Correlations in controls were not significant (not shown). Conclusion Eotaxin-3 gene expression is upregulated in the airway epithelium in eosinophilic asthma and highly correlates with IL-13 signature genes – including biomarker genes POSTN and NOS2. Please refer to page A190 for declarations of interest related to this abstract.
Source: Thorax
Authors: S Couillard, J Melhorn, A Singhania, D Horowitz, R Djukanovíc, CH Woelk, TSC Hinks
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W3213653817