Work

Cubilin-, megalin-, and Dab2-dependent transcription revealed by CRISPR/Cas9 knockout in kidney proximal tubule cells

Year: 2021

Type: article

Abstract: Megalin and cubilin receptors together with their adaptor protein Dab2 represent major components of the endocytic machinery responsible for efficient uptake of filtered proteins by the proximal tubule (PT). Dab2 and megalin expression have been implicated as both positive and negative modulators of kidney disease. We used RNA sequencing to knock out CRISPR/Cas9 cubilin, megalin, and Dab2 in highly differentiated PT cells to identify PT-specific changes that are directly consequent to knockout of each component.

Source: American Journal of Physiology-Renal Physiology

DOI: 10.1152/ajprenal.00259.2021

Authors: Kimberly R. Long, Youssef Rbaibi, Corry D. Bondi, B. Rhodes Ford, Amanda C. Poholek, Cary R. Boyd-Shiwarski, Roderick J. Tan, Joseph D. Locker, Ora A. Weisz

Institution: University of Pittsburgh

Language: English

Topic: Renal Diseases and Glomerulopathies

Subfield: Nephrology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: American Heart Association, American Society of Nephrology, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: G1019732981, G2211430106, G2250247325, G2339487983, G2590533900, The role of MCP-1 in tubular-to-glomerular crosstalk in proteinuric kidney disease, Pittsburgh Center for Kidney Research, The function of kidney specific (KS)-WNK1 condensates during potassium stress, Lung-specific expression and function of Blimp-1 in T cells impacting allergic asthma

OpenAlex ID: W3214152016

FWCI: 3.181

Cites: 68

Cited by: 29

Related to: 10