Work

Macro photography with Lighsheet Illumination Enables Whole Expanded Brain Imaging with Single-cell Resolution

Year: 2021

Type: article

Abstract: Macro photography allows direct visualization of the enlarged whole mouse brain by a combination of lightsheet illumination and expansion microscopy with single-cell resolution. Taking advantage of the long working distance of a camera lens, we imaged a 3.7 cm thick, transparent, fluorescently-labeled expanded brain. In order to improve 3D sectioning capability, we used lightsheet excitation confined as the depth of field of the camera lens. Using 4x sample expansion and 5x optical magnification, macro photography enables imaging of expanded whole mouse brain with an effective resolution of 300 nm, which provides the subcellular structural information at the organ level.

Source: Discoveries

DOI: 10.15190/d.2021.12

Authors: Chia-Ming Lee, Xuejiao Tian, Chieh Tsao, Peilin Chen, Tzyy-Nan Huang, Yi-Ping Hsueh, Bi-Chang Chen

Institutions: Research Center for Applied Science, Academia Sinica, Academia Sinica, National Tsing Hua University, Institute of Molecular Biology, Academia Sinica, Institute of Cellular and Organismic Biology, Academia Sinica

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

OpenAlex ID: W3215286941

FWCI: 0.567

Cites: 10

Cited by: 7

Related to: 10