Plug-and-play polymer microfluidic chips for hydrated, room temperature, fixed-target serial crystallography
Year: 2021
Type: article
Abstract: protein crystallization (to eliminate crystal handling) or crystal slurry loading, with prepared samples stable for weeks in a humidified environment and for several hours in ambient conditions. Serial oscillation crystallography, using a multi-crystal rotational data collection approach, at a microfocus synchrotron beamline (SSRL, beamline 12-1) was used to benchmark the performance of the chips. High-resolution structures (1.3-2.7 Å) were collected from five different proteins - hen egg white lysozyme, thaumatin, bovine liver catalase, concanavalin-A (type VI), and SARS-CoV-2 nonstructural protein NSP5. Overall, our modular fabrication approach enables precise control over the cross-section of materials in the X-ray beam path and facilitates chip adaption to different sample and beamline requirements for user-friendly, straightforward diffraction measurements at room temperature.
Source: Lab on a Chip
DOI: 10.1039/d1lc00810b
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: green
Awards: G14554683, Technology Development for Biological Imaging with XFELs, G303061748, Center for Biologically Inspired Nano-scaffolds for Mitigating Chlamydia trachomatis Pathogenesis, Synthetic Generation of a Chlamydia Vaccine, A Synchrotron Radiation Structural Biology Resource, Biology with X-ray Lasers
OpenAlex ID: W3216060418