Antibiofilm Activity of Human Milk Oligosaccharides against Multidrug Resistant and Susceptible Isolates of Acinetobacter baumannii
Year: 2021
Type: article
Abstract: Acinetobacter baumannii is a serious threat to human health, per the Centers for Disease Control and Prevention’s latest threat assessment. A. baumannii is a Gram-negative opportunistic bacterial pathogen that causes severe community and nosocomial infections in immunocompromised patients. Treatment of these infections is confounded by the emergence of multi- and pan-drug resistant strains of A. baumannii . A. baumannii colonizes abiotic and biotic surfaces and evades antimicrobial challenges by forming biofilms, which are three-dimensional architectural structures of cells adhered to a substrate and encased in an extracellular matrix comprised of polymeric substances such as polysaccharides, proteins, and DNA. Biofilm-inhibiting compounds have recently gained attention as a chemotherapeutic strategy to prevent or disperse A. baumannii biofilms and restore the utility of traditional antimicrobial strategies. Recent work indicates that human milk oligosaccharides (HMOs) have potent antibacterial and biofilm-inhibiting properties. We sought to test the utility of HMOs against a bank of clinical isolates of A. baumannii to ascertain changes in bacterial growth or biofilm formation. Our results indicate that out of 18 strains tested, 14 were susceptible to the antibiofilm activities of HMOs, and that the potent antibiofilm activity was observed in strains isolated from diverse anatomical sites, disease manifestations, and across antibiotic-resistant and susceptible strains.
Source: ACS Infectious Diseases
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: U.S. Department of Veterans Affairs, Center for Selective C-H Functionalization, National Science Foundation, National Institutes of Health, National Heart, Lung, and Blood Institute, National Eye Institute, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Research Resources, Center for Scientific Review, National Center for Advancing Translational Sciences, National Institute on Minority Health and Health Disparities, Eunice Kennedy Shriver National Institute of Child Health and Human Development
Awards: G1523334746, G1624507791, G1710084388, G223019220, G2503450702, G2750699093, Meharry Clinical and Translational Research Center (MeTRC), G3964786795, Vanderbilt Mouse Metabolic Phenotyping Center, G4119542496, G4484724989, Training in Cardiovascular Research, G5357857338, Determining the contribution of zinc deficiency to perinatal Group B Streptococcus infections, The Vanderbilt Institute for Clinical and Translational Research (VICTR) UL1, G7357279838, The Vanderbilt Institute for Clinical and Translational Research (VICTR), G8250921216, G8322142740, Molecular and Cellular Basis for Digestive Diseases, Bioorganic Approaches Toward Novel Antimicrobial Agents and Chemotherapeutics, Initiative for Maximizing Student Development (IMSD) at Vanderbilt, Cancer Center Support Grant, Vanderbilt Diabetes Research Center, Core Grant in Vision Research, G926929692
OpenAlex ID: W3216367986