Year: 2014
Type: dataset
Abstract: Measurement of gene expression usually involves isolation of total RNA which includes mRNAs.Isolation of high quality intact RNA from the pancreas can be a challenge because of the very high levels of RNase.This protocol describes modifications to the widely used guandinium thiocyanate-phenol-chloroform extraction method, using commercial preparations of extraction reagent.The major modification to existing protocols is to increase the ratio of extraction reagent to tissue wet weight.This procedure yields high quality total RNA suitable for downstream measurement of gene expression such as quantitative RT-PCR and microarray transcriptome analysis.
DOI: 10.3998/panc.2014.9
Author: Robert C. De Lisle
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W322155408