Automated Microscopy and Image Analysis for Androgen Receptor Function
Year: 2011
Type: book-chapter
Abstract: Systems-level approaches have emerged that rely on analytical, microscopy-based technology for the discovery of novel drug targets and the mechanisms driving AR signaling, transcriptional activity, and ligand independence. Single cell behavior can be quantified by high-throughput microscopy methods through analysis of endogenous protein levels and localization or creation of biosensor cell lines that can simultaneously detect both acute and latent responses to known and unknown androgenic stimuli. The cell imaging and analytical protocols can be automated to discover agonist/antagonist response windows for nuclear translocation, reporter gene activity, nuclear export, and subnuclear transcription events, facilitating access to a multiplex model system that is inherently unavailable through classic biochemical approaches. In this chapter, we highlight the key steps needed for developing, conducting, and analyzing high-throughput screens to identify effectors of AR signaling.
Source: Methods in molecular biology
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G2169131389, Center for Reproductive Biological Research, Multidisciplinary K12 Urologic Research (KURe) Career Development Program, Unique Roles of p160 Coactivators during Human Adipogenesis, Steroid Receptor Function and Nuclear Organization, Reproductive Biology: Regulatory Mechanisms, Texas Medical Center Digestive Diseases Center, Baylor College of Medicine Cancer Center - Cancer Center Support Grant
OpenAlex ID: W33672729