Work

Characterization of daptomycin non-susceptible Enterococcus faecium producing urinary tract infection in a renal transplant recipient.

Year: 2015

Type: article

Abstract: OBJECTIVES: Characterization of a urine isolate of daptomycin non-susceptible Enterococcus faecium recovered from a patient with kidney transplantation and no history of daptomycin exposure. METHODS: After isolation in a urine sample, identification of E. faecium was confirmed by amplification of the E. faecium-specific gene encoding D-alanyl-D-alanine ligase (ddl) and daptomycin susceptibility testing was performed by E-test on cation-adjusted Mueller-Hinton agar. In order to determine the genetic bases of daptomycin resistance, the open reading frames of five genes previously associated with daptomycin resistance in enterococci were sequenced. RESULTS: Substitutions in the response regulator LiaR (S19F) and cardiolipin synthase (R218Q) were identified. CONCLUSIONS: To the best of our knowledge, this is the first characterization of emerging daptomycin resistance in E. faecium in a Spanish hospital in the absence of daptomycin exposure and in a renal transplant recipient.

Source: PubMed

Authors: Antonio Sorlózano, Diana Panesso, José María Navarro-Marí, Cesar A Arias, José Gutiérrez-Fernández

Institution: Universidad de Granada

Language: English

Topic: Antibiotic Resistance in Bacteria

Subfield: Microbiology (medical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Award: Molecular Mechanisms Of Daptomycin Resistance In Enterococci