Year: 2021
Type: article
Abstract: To better understand the input-output computations of neuronal populations, we developed ArcLight-ST, a genetically-encoded voltage indicator, to specifically measure subthreshold membrane potentials. We combined two-photon imaging of voltage and calcium, and successfully discriminated subthreshold inputs and spikes with cellular resolution in vivo. We demonstrate the utility of the method by mapping epileptic seizures progression through cortical circuits, revealing divergent sub- and suprathreshold dynamics within compartmentalized epileptic micronetworks. Two-photon, two-color imaging of calcium and voltage enables mapping of inputs and outputs in neuronal populations in living animals.
Source: Nature Communications
Authors: Yuki Bando, Michael Wenzel, Rafael Yuste
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,560
Funders: U.S. Department of Health and Human Services, Takeda Science Foundation, Uehara Memorial Foundation, Hamamatsu Foundation for Science and Technology Promotion, Deutsche Forschungsgemeinschaft, Ministry of Education, Culture, Sports, Science and Technology, Japan Science Society, Multidisciplinary University Research Initiative, Japan Society for the Promotion of Science, National Institute of Mental Health, National Eye Institute, National Institute of Neurological Disorders and Stroke, Army Research Office, Army Research Laboratory
Awards: Optical methods for imaging and manipulating dendritic spines in vivo, G1242600127, G2723399458, G3079858989, G4012432327, Regulation of neocortical development with leak sodium channels., G4544234697, Functional consequences of GABAergic inhibition of dendritic spines at puberty, G5259331294, G6304086971, Imaging Functional Connectivity in Visual Cortex, CRCNS: Modeling the nanophysiology of dendritic spines, Functional connectomics of the neocortical microcircuit, G6979580094, G7452299184, G7518383307, G8578859301, G8602449355, Two-photon optical control of astrocytic function
OpenAlex ID: W4200222178