Work

Common computational tools for analyzing CRISPR screens

Year: 2021

Type: review

Abstract: CRISPR-Cas technology offers a versatile toolbox for genome editing, with applications in various cancer-related fields such as functional genomics, immunotherapy, synthetic lethality and drug resistance, metastasis, genome regulation, chromatic accessibility and RNA-targeting. The variety of screening platforms and questions in which they are used have caused the development of a wide array of analytical methods for CRISPR analysis. In this review, we focus on the algorithms and frameworks used in the computational analysis of pooled CRISPR knockout (KO) screens and highlight some of the most significant target discoveries made using these methods. Lastly, we offer perspectives on the design and analysis of state-of-art multiplex screening for genetic interactions.

Source: Emerging Topics in Life Sciences

DOI: 10.1042/etls20210222

Authors: Medina Colic, Traver Hart

Institutions: The University of Texas MD Anderson Cancer Center, The University of Texas Health Science Center at Houston

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: Cancer Prevention and Research Institute of Texas, Andrew Sabin Family Foundation, National Cancer Institute, National Institute of General Medical Sciences

Awards: Recruitment of First-Time, Tenure-Track Faculty Members, G7534854087, Deciphering the hierarchical modularity of the mammalian cell through network integration and complex genetic perturbation strategies, Cancer Center Support Grant