Corrigendum: Identification and Characterization of Thermostable Y-Family DNA Polymerases η, ι, κ and Rev1 From a Lower Eukaryote, Thermomyces lanuginosus
Year: 2021
Type: erratum
Abstract: Corrigendum on: Vaisman A, McDonald JP, Smith MR, Aspelund SL, Evans TC and Woodgate R (2021) Identification and Characterization of Thermostable Y-Family DNA Polymerases η, ι, κ and Rev1 From a Lower Eukaryote, Thermomyces lanuginosus. Front. Mol. Biosci. 8:778400. doi: 10.3389/fmolb.2021.778400In the original article, there was a formatting issue in Figure 6 as published. This occurred when the image was converted from a PC generated .pdf to an Apple Macintosh generated .tif for publication. The corrected Figure 6 appears below. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. . The substrate used in these assays was made by annealing of the 32 P labeled primer 5ʹ-CACTGCAGACTCTAAA-3ʹ and either an undamaged or BPDE-containing template 5ʹ-GCTCGTCAGCAGATTTAGAGTCTGCAGTG-3ʹ, where the underlined bold A stands for the undamaged, or BPDE modified dA. Reactions contained 100 μM each individual nucleotide (dC, dG, dA, dT) or mixture of all four dNTPs as indicated in the figure and were carried out at 37°C for 10 min. Concentrations of enzymes were 0.17 pM for polη, 0.32 pM for polκ, and 0.15 pM for polι. The sequence of the template immediately downstream of the primer (pr) is shown on the left-hand side of each gel pair. The star (*) indicates the position of the adduct.
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,950
OpenAlex ID: W4200267320