Work

Structure of anchorless RML prion reveals motif variation between strains

Year: 2021

Type: preprint

Abstract: Little is known about the structural basis of prion strains. Here we provide a high (3.0 Å) resolution cryo-electron microscopy-based structure of brain-derived fibrils of the mouse anchorless RML scrapie strain which, like the recently determined hamster 263K strain, has a parallel in-register β-sheet-based core. However, detailed comparisons reveal that variations in shared structural motifs provide a basis for prion strain determination. One-sentence summary Cryo-electron microscopy reveals a near-atomic structure of an infectious, brain-derived murine prion fibril and strain differences.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.1101/2021.12.22.473909

Authors: Forrest Hoyt, Heidi G. Standke, Efrosini Artikis, Cindi L. Schwartz, Bryan Hansen, Kunpeng Li, Andrew G. Hughson, Matteo Manca, Olivia R. Thomas, Gregory J. Raymond, Gerald S. Baron, Byron Caughey, Allison Kraus

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases, Case Western Reserve University

Language: English

Topic: Prion Diseases and Protein Misfolding

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Britton Fund, Case Western Reserve University, National Institutes of Health, National Institute of Allergy and Infectious Diseases

OpenAlex ID: W4200326770

Cites: 24

Cited by: 10

Related to: 10