A Bivalent Activatable Fluorescent Probe for Screening and Intravital Imaging of Chemotherapy‐Induced Cancer Cell Death
Year: 2021
Type: article
Abstract: The detection and quantification of apoptotic cells is a key process in cancer research, particularly during the screening of anticancer therapeutics and in mechanistic studies using preclinical models. Intravital optical imaging enables high-resolution visualisation of cellular events in live organisms; however, there are few fluorescent probes that can reliably provide functional readouts in situ without interference from tissue autofluorescence. We report the design and optimisation of the fluorogenic probe Apotracker Red for real-time detection of cancer cell death. The strong fluorogenic behaviour, high selectivity, and excellent stability of Apotracker Red make it a reliable optical reporter for the characterisation of the effects of anticancer drugs in cells in vitro and for direct imaging of chemotherapy-induced apoptosis in vivo in mouse models of breast cancer.
Source: Angewandte Chemie
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $5,000
Funders: AstraZeneca, Fundación Alfonso Martín Escudero, Wellcome Trust, Cold Spring Harbor Laboratory, Ministerio de Ciencia, Innovación y Universidades, Thompson Family Foundation, Northwell Health, Royal Society, Ministerio de Ciencia e Innovación, National Institutes of Health, Engineering and Physical Sciences Research Council, European Research Council, National Cancer Institute
OpenAlex ID: W4200439419