Work

Miniature structured illumination microscope for in vivo 3D imaging of brain structures with optical sectioning

Year: 2022

Type: article

Abstract: We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 µm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 µm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 µm in the brain.

Source: Biomedical Optics Express

DOI: 10.1364/boe.449533

Authors: Omkar D. Supekar, Andrew Sias, Sean R. Hansen, Gabriel Martinez, Graham C. Peet, Xiaoyu Peng, Victor M. Bright, Ethan G. Hughes, Diego Restrepo, Douglas P. Shepherd, Cristin G. Welle, Juliet T. Gopinath, Emily A. Gibson

Institutions: University of Colorado Boulder, University of Colorado Anschutz, Arizona State University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: National Institutes of Health, University of Colorado Boulder, Anschutz Medical Campus, University of Colorado, National Eye Institute, National Institute of Neurological Disorders and Stroke

Awards: G3229208718, 3D-Fast Optical Interface for Rapid Volumetric Neural Sensing and Modulation, Shedding light on brain circuits mediating navigation of the odor plume in a natural environment, Development of 3D-FAST Optical Interface for Rapid Volumetric Neural Sensing and Modulation

OpenAlex ID: W4220882506

FWCI: 4.74

Cites: 38

Cited by: 46

Related to: 10