Work

Functional phenotyping of circulating human cytotoxic T cells and NK cells using a 16-color flow cytometry panel

Year: 2021

Type: article

Abstract: Cytotoxic T lymphocytes and natural killer (NK) cells are key effector cells in immune defenses against intracellular pathogens and cancer. In human blood, effector T and NK cytotoxic cells comprise a diverse and relatively rare group of cells. Herein, we describe a simplified intracellular staining workflow for classification of circulating human T and NK cells with cytolytic potential. We suggest reagents for measuring cytolytic proteins and identification of cell subsets within conventional and unconventional T cells and NK cells.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2021.101069

Authors: Gisele V. Baracho, Nihan Kara, Stephanie Rigaud, Evelyn Lo, Stephanie J. Widmann, Aaron J. Tyznik

Institution: BD Biosciences (United States)

Language: English

Topic: Immune Cell Function and Interaction

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500