Work

Acquisition, processing, and single-cell analysis of normal human breast tissues from a biobank

Year: 2021

Type: article

Abstract: The Komen Tissue Bank is the only biorepository in the world for normal breast tissues from women. Below we report the acquisition and processing of breast tissue from volunteer donors and describe an experimental and analysis pipeline to generate a single-cell atlas. This atlas is based on single-cell RNA-seq and is useful to derive breast epithelial cell subcluster-specific gene expression signatures, which can be applied to breast cancer gene expression data to identify putative cell-of-origin. For complete details on the use and execution of this protocol, please refer to Bhat-Nakshatri et al. (2021).

Source: STAR Protocols

DOI: 10.1016/j.xpro.2021.101047

Authors: Poornima Bhat-Nakshatri, Natascia Marino, Hongyu Gao, Yunlong Liu, Anna Maria Storniolo, Harikrishna Nakshatri

Institutions: Indiana University School of Medicine, Indiana University Indianapolis, Indiana University Melvin and Bren Simon Comprehensive Cancer Center, Richard L. Roudebush VA Medical Center

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,500