Work

Faculty Opinions recommendation of Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine target.

Year: 2009

Type: peer-review

Abstract: Broadly neutralizing antibodies (bNAbs), which develop over time in some HIV-1 infected individuals, define critical epitopes for HIV vaccine design.Using a systematic approach, we have examined neutralization breadth in the sera of about 1,800 HIV-1 infected individuals, primarily infected with non-clade B viruses, and selected donors for monoclonal antibody (mAb) generation.We then used a high-throughput neutralization screen of antibody-containing culture supernatants from approximately 30,000 activated memory B cells from a clade A-infected African donor to isolate two potent mAbs that target a broadly neutralizing epitope.The previously undescribed epitope is preferentially expressed on trimeric Envelope protein and spans conserved regions of variable loops of the gp120 subunit.The results provide a framework for the design of new vaccine candidates for the elicitation of bNAb responses.

Source: Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature

DOI: 10.3410/f.1163775.625621

Authors: Suzanne Crowe, Paul Gorry

Institution: Burnet Institute

Language: English

Topic: HIV Research and Treatment

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: United States Agency for International Development, Bill and Melinda Gates Foundation, International AIDS Vaccine Initiative, Thailand Science Research and Innovation, National Institutes of Health

OpenAlex ID: W4205113281

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