10X Genomics Single-Nucleus Assay of Transposase Accessible Chromatin-Sequencing for Epigenetic Profiling of Adult Human Tissues v1
Year: 2021
Type: preprint
Abstract: 10x Genomics Single Cell ATAC (v1) sequencing is a microdroplet-based method that allows for the effective capture, sequencing and profiling of accessible chromatin in single nuclei. Chromatin accessibility is a major determinant of gene regulation, defining the transcriptional regulatory networks that determine cellular identity and function as well as additional biological processes (e.g., differentiation, proliferation, development and responses to the extracellular environment). Chromatin accessibility sequencing (ATAC-seq) provides insight into the upstream regulatory landscape associated with open and accessible chromatin regions. References 1. Lake et al. (2016). Science, doi:10.1126/science.aaf1204. 2. Lake et al. (2018). Nature Biotechnology, doi:10.1038/nbt.4038. 3. Lake et al. (2019). Nature Communications, doi:10.1038/s41467-019-10861-2. 4. Chromium Single Cell ATAC Reagent Kits User Guide v1 (Rev B) CG000168, support.10xgenomics.com. 5. Lake and Zhang. Nuclei Isolation Protocol Applicable to Adult Human Kidney Tissues, kpmp.org/researcher-resources.
Authors: Blue Lake, Dinh Diep, Kun Zhang, Sarah Urata
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4205217169