Work

RNA extraction from Sterivex using phenol:chloroform v1

Year: 2020

Type: preprint

Abstract: This extraction protocol uses bead-beating techniques within the Sterivex to dislodge biomass from the Sterivex filter. Biomass in solution is then removed from the Sterivex and RNA is extracted using acid phenol and chloroform. The protocol has been tested and used on eutrophic freshwater samples dominated by toxic cyanobacteria (Microcystis).

DOI: 10.17504/protocols.io.bhu6j6ze

Authors: Helena Pound, Steven W Wilhelm

Language: English

Topic: Aquatic Ecosystems and Phytoplankton Dynamics

Subfield: Environmental Chemistry

Field: Environmental Science

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W4205466630

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Cited by: 2

Related to: 10