Work

Patient-specific, re-engineered cardiomyocyte model confirms the circumstance-dependent arrhythmia risk associated with the African-specific common SCN5A polymorphism p.S1103Y: Implications for the increased sudden deaths observed in black individuals during the COVID-19 pandemic

Year: 2021

Type: article

Abstract: Background During the early stages of the coronavirus disease 2019 (COVID-19) pandemic, a marked increase in sudden cardiac death (SCD) was observed. The p.S1103Y-SCN5A common variant, which is present in ∼8% of individuals of African descent, may be a circumstance-dependent, SCD-predisposing, proarrhythmic polymorphism in the setting of hypoxia-induced acidosis or QT-prolonging drug use. Objective The purpose of this study was to ascertain the effects of acidosis and hydroxychloroquine (HCQ) on the action potential duration (APD) in a patient-specific induced pluripotent stem cell–derived cardiomyocyte (iPSC-CM) model of p.S1103Y-SCN5A. Methods iPSC-CMs were generated from a 14-year-old p.S1103Y-SCN5A-positive African American male. The patient's variant-corrected iPSC-CMs (isogenic control [IC]) were generated using CRISPR/Cas9 technology. FluoVolt voltage-sensitive dye was used to assess APD 90 values in p.S1103Y-SCN5A iPSC-CMs compared to IC before and after an acidotic state (pH 6.9) or 24 hours of treatment with 10 μM HCQ. Results Under baseline conditions (pH 7.4), there was no difference in APD 90 values of p.S1103Y-SCN5A vs IC iPSC-CMs ( P = NS). In the setting of acidosis (pH 6.9), there was a significant increase in fold-change of APD 90 in p.S1103Y-SCN5A iPSC-CMs compared to IC iPSC-CMs ( P <.0001). Similarly, with 24-hour 10 μM HCQ treatment, the fold-change of APD 90 was significantly higher in p.S1103Y-SCN5A iPSC-CMs compared to IC iPSC-CMs ( P <.0001). Conclusion Although the African-specific p.S1103Y-SCN5A common variant had no effect on APD 90 under baseline conditions, the physiological stress of either acidosis or HCQ treatment significantly prolonged APD 90 in patient-specific, re-engineered heart cells.

Source: Heart Rhythm

DOI: 10.1016/j.hrthm.2021.12.029

Authors: Samantha K. Hamrick, C.S. John Kim, David J. Tester, John R. Giudicessi, Michael J. Ackerman

Institution: Mayo Clinic

Language: English

Topic: Cardiac electrophysiology and arrhythmias

Subfield: Cardiology and Cardiovascular Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,330