Faculty Opinions recommendation of A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization.
Year: 2004
Type: peer-review
Abstract: In yeast Saccharomyces cerevisiae, Ash1p, a protein determinant for mating-type switching, is segregated within the daughter cell nucleus to establish asymmetry of HO expression.The accumulation of Ash1p results from ASH1 mRNA that is sorted as a ribonucleoprotein particle (mRNP or locasome) to the distal tip of the bud where translation occurs.To study the mechanism regulating ASH1 mRNA translation, we isolated the ASH1 locasome and characterized the associated proteins by MALDI-TOF.One of these proteins was Puf6p, a new member of the PUF family of highly conserved RNA-binding proteins such as Pumilio in Drosophila, responsible for translational repression, usually to effect asymmetric expression.Puf6p-bound PUF consensus sequences in the 3UTR of ASH1 mRNA and repressed the translation of ASH1 mRNA both in vivo and in vitro.In the puf6⌬ strain, asymmetric localization of both Ash1p and ASH1 mRNA were significantly reduced.We propose that Puf6p is a protein that functions in the translational control of ASH1 mRNA, and this translational inhibition is necessary before localization can proceed.
Author: Roy Long
Institution: Medical College of Wisconsin
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funder: National Institutes of Health
OpenAlex ID: W4205903566