The Influence of plant growth regulators and slicing methods inducing direct organogenesis of meristematic florets of date palm
Year: 2020
Type: article
Abstract: In date palm micropropagation protocols, the female inflorescences play an important role, being available tissue culture-explants, alternatively of the limited number of renewing offshoots explants of date palm that used for in vitro propagation.This study conducted to investigate the effect of different auxins types (2,4-D, NAA, NOA) at different concentrations, supplemented in induction culture medium of constant concentration of cytokinins, on inducing the direct organogenesis by adventitious shoots differentiation, of the date palm immature female inflorescences explants, of Barhe cultivar.Also, the interaction effect of slicing method (for the first time studying) of the starting spikelet explants was studied.The results showed that, the induction medium of the appropriate ratio of the NAA auxin type to cytokinins of induction medium, with applying the slicing method of fine chopping for the cultured spikelet, showed the most satisfying results, of direct adventitious shoots formation, direct somatic embryos, and less formation of direct adventitious roots.Developing the date palm micropropagation protocol by inflorescences explants considered a reliable and rapid opportunity to avoid the shortage of date palm offshoots and covering the commercial demands on the date palm plantlets.
Language: English
Topic: Date Palm Research Studies
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W4205953736