Year: 2022
Type: article
Abstract: AIM: Penicillin-induced epileptiform activity is widely used as an acute seizure model.The 5-HT7 receptor is the most recently described 5-HT receptor member.Oxidative stress affects many physiological mechanisms, including epilepsy.The study aims to investigate the effect of 5-HT7 serotonin receptor on penicillininduced epileptiform activity and the role of reactive oxygen species in this effect by electrophysiological and biochemical methods.METHODS: In this study, male albino Wistar rats (180-240g) were used (each group n:6).30 minutes after induction of epilepsy via penicillin, agonist of 5-HT7, AS 19 (1,25, 0,625 and 0,3125 μg/rat) doses and selective-antagonist of 5-HT7, SB 269970 (2.5, 1,25 and 0,625 μg/rat) doses were administered intracerebroventricularly. The ECOG recordings were analyzed for spike frequency and amplitude.After the effective doses of AS 19 and SB 269970 were determined, their combinations were also applied intracerebroventricularly.Malondialdehyde, superoxide dismutase, catalase and glutathione levels were measured by ELISA method in the plasma and tissue samples from different brain regions.One-way analysis of variance and Post Hoc Tukey tests were used for statistical analysis.This study was approved by the local ethics committee (HADYEK, 2017/55).This project was supported by TÜBİTAK (Project Number: 119S194).RESULTS: Compared to the control group, 0,625 μg dose of AS 19 significantly reduced the spike frequency (p<0.01).1,25 μg dose of SB 269970 significantly increased spike frequency (p<0.05).When 0,625 μg AS 19 and 1,25 μg SB 269970 were given together, the spike frequency decreased significantly (p<0.05).AS 19 decreased oxidative stress parameters (p<0.05),but not SB 269970 (p>0.05).CONCLUSION: AS 19 showed the anticonvulsant effect on penicillin-induced epileptiform activity and blocked the proconvulsive effect of SB 269970.To elucidate the detailed physiological mechanism of this result, it needs further molecular studies.
Source: Acta Physiologica
DOI: 10.1111/apha.13753
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W4206345609