Optimizing procedures for a human genome repository
Year: 1989
Type: report
Abstract: Repositories of well-characterized cloned human DNA fragments currently exist, but at a smaller scale than necessary for any genomic project. Procedures used in these repositories cannot be expanded without modification. Automation must be incorporated wherever feasible, including such activities as DNA preparation, clone verification, data maintenance and analysis, and sample storage, recovery, and distribution. Methods to reduce the amount of sample needed for verification and storage must also be perfected. The objective of this project has been to develop and evaluate protocols and instrumentation while establishing a pilot repository on a genomic scale. Initial emphasis has been placed on automating clone verification by analyzing restriction fragments on a DNA sequencing machine and comparing fragment sizes to those already obtained by depositors. Intrinsic to this development has been testing sample preparation, preservation, and information management. So far, these procedures have been tested using the contigs of lambda clones identified by Maynard Olson's laboratory for the {ital Saccharomyces cerevisiae} genome. 7 refs., 6 figs., 5 tabs.
DOI: 10.2172/5673311
Author: W. Nierman
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4206440137