Work

Efficient CRISPR-Cas9 editing of major evolutionary loci in sticklebacks.

Year: 2019

Type: article

Abstract: BACKGROUND: Stickleback fish are widely used to study the genetic and ecological basis of phenotypic evolution. Although several major loci have now been identified that contribute to evolutionary differences between wild populations, further study of the phenotypes associated with particular genes and mutations has been limited by the difficulty of generating targeted mutations at precise locations in the stickleback genome. APPROACH AND AIMS: gene would disrupt the formation of most armor plates, fin rays, spines, teeth, and gill rakers. RESULTS: mutations. CONCLUSION: in skin pigmentation and armor plate reduction, respectively. They also provide new information about the functions of these genes in other body structures.

Source: PubMed

Authors: Julia I Wucherpfennig, Craig T Miller, David M Kingsley

Institutions: Stanford Medicine, Stanford University, University of California, Berkeley, Howard Hughes Medical Institute

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Dental and Craniofacial Research

Awards: Genetics and Developmental Biology Training Program, Development Genetics of Tooth Number Variation in Sticklebacks

OpenAlex ID: W4206866113

FWCI: 1.099

Cites: 69

Cited by: 23

Related to: 10