Year: 2021
Type: article
Abstract: SUMMARY We have used a combination of chemical genetics, chromatin proteomics and imaging to map the earliest chromatin transactions during vertebrate cell entry into mitosis. Chicken DT40 CDK1 as cells undergo synchronous mitotic entry within 15 minutes following release from a 1NM-PP1-induced arrest in late G 2 . In addition to changes in chromatin association with nuclear pores and the nuclear envelope, earliest prophase is dominated by changes in the association of ribonucleoproteins with chromatin, particularly in the nucleolus, where pre-rRNA processing factors leave chromatin significantly before RNA polymerase I. Nuclear envelope barrier function is lost early in prophase and cytoplasmic proteins begin to accumulate on the chromatin. As a result, outer kinetochore assembly appears complete by nuclear envelope breakdown (NEBD). Most interphase chromatin proteins remain associated with chromatin until NEBD, after which their levels drop sharply. An interactive proteomic map of chromatin transactions during mitotic entry is available as a resource at https://mitoChEP.bio.ed.ac.uk .
Source: Molecular Cell
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $8,900
Funders: Wellcome Trust, Medical Research Council
Awards: The role of non-histone proteins in chromosome structure and function during mitosis, The role of non-histone proteins in chromosome structure and function during mitosis., G6574036170, How cells regulate protein levels (and fail to do so in cancer), The role of non-histone proteins in chromosome structure and function during mitosis., Core Funding for the Wellcome Trust Centre for Cell Biology
OpenAlex ID: W4210330911