Work

Covalent and Non-Covalent Strategies for the Immobilization of Tobacco Etch Virus (TEV) Protease on Superparamagnetic Nanoparticles

Year: 2019

Type: preprint

Abstract: TEV protease fusion proteins were prepared and immobilized on superparamagnetic nanoparticles (SPIONs). Non-covalent (avidin-biotin) and covalent (HALOtag-chloroalkane) strategies were explored for TEV immobilization. HALOtag immobilized TEV protease provided enhance performance in both enzyme retention and reusability. SPION-immobilized proteases could be a convenient and cost-saving tool in the life sciences

Source: ChemRxiv

DOI: 10.26434/chemrxiv.11365616

Authors: Jessica L. Norris, Tulsi Patel, Anvesh K. R. Dasari, Thomas A. Cope, Kwang Hun Lim, Robert Hughes

Institution: East Carolina University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: American Chemical Society Petroleum Research Fund

Award: G2757699074