Corrigendum: Platelet-Derived Growth Factor-D Activates Complement System to Propagate Macrophage Polarization and Neovascularization
Year: 2022
Type: erratum
Abstract: In the original article, there was an error. In typesetting, the texts under the subtitle RNA Isolation and Real Time quantitative PCR from Materials and methods part were accidentally overwritten by the content under subtitle RNA sequencing and Transcriptome Analysis.A correction has been made to Materials and methods, RNA isolation and real time quantitative PCR, Paragraph 1: Total RNA was isolated using the TRNzol reagent (TIANGEN, cat: DP424) and converted to cDNA using the Fast King RT Kit (TIANGEN, cat: KR116) according to the manufacturer's instructions. Real-time quantitative PCR was carried out in a 10 μL reaction containing the SYBR Select Master Mix (Vazyme, cat: Q331) in technical quadruplicate using a Quantstudio 6K Flex system (Life Technologies). Results were analyzed using the Quantstudio 12 K Flex Software v1.2.2 (Thermo Fisher Scientific). Relative mRNA levels were calculated based on the 2− ∆∆C T method, using the 18S rRNA as references.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,950
OpenAlex ID: W4210909257